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Vaccination & Diagnostics

This section contains materials relating to vaccination, diagnostics and laboratories, and research.  The technical material available will be expanded in accordance with the needs of technical professionals utilising the toolkit.  To suggest additional topics or submit items for the technical corner, please email: support@aitoolkit.org

 

This includes:

  • factors to consider in using vaccination as an aid for control and eradication
  • OIE Avian Influenza: Manual of Diagnostic tests and Vaccines for Terrestrial Animals 2008
  • information on the development, production and use of vaccines
  • scientific, economic, regulatory and socio-ethical aspects of animal vaccine use
    information on sample shipping
  • information relating to vaccination for avian influenza in Asia.

Case Studies

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    A team at the National University of Singapore, Velumani et al has developed a peptide enzyme-linked immunosorbent assay (ELISA) to detect infection with H5N1 avian influenza in humans and animals based an H5 HA epitope that is conserved 100% in human H5N1 isolates and 97% in avian isolates. The team evaluated the sensitivity and specificity of the assay using chicken antisera to H5N1 viruses from divergent clades and other influenza viruses, as well as human sera from patients infected with H5N1 or seasonal influenza. The assay detected antibodies to H5N1 more accurately than hemagglutinin inhibition, immunofluorescence assay, and immunodot blot tests.

  • Avian Influenza Vaccination in Egypt

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    10/02/2010 1:19:03 PM
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    The decision to use mass vaccination against HPAI in Egypt was taken as an emergency measure – however, three years on, the impact has been very limited. This study discusses the limitations of the current vaccination strategy in Egypt.

  • Avian influenza vaccination: the experience in China

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    25/02/2010 12:55:47 PM
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    This study, prepared for the OIE Scientific and Technical Review series by H. Chen, examines China’s vaccination strategy to control avian influenza infection in poultry including development, implementation, challenges and efficacy.

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    This work was supported by grants from the University of Bologna. The study examines whether preen oil gland secretions, by which all aquatic birds make their feathers waterproof, support a natural mechanism that concentrates avian influenza viruses from water onto bird bodies, therefore posing a possible source of infection.

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    The neuraminidase (NA) inhibitors oseltamivir and zanamivir are the first-line of defense against potentially fatal variants of influenza A pandemic strains. However, if resistant virus strains start to arise easily or at a high frequency, a new antiinfluenza strategy will be necessary. This study by Orozovic et al aimed to investigate if and to what extent NA inhibitor–resistant mutants exist in the wild population of influenza A viruses that inhabit wild birds.

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    Oseltamivir (Tamiflu®) is the most widely used drug against influenza infections and is extensively stockpiled worldwide as part of pandemic preparedness plans. However, resistance is a growing problem. The active metabolite of oseltamivir, oseltamivir carboxylate (OC), is poorly degraded in sewage treatment plants and surface water and has been detected in aquatic environments where the natural influenza reservoir, dabbling ducks, can be exposed to the substance. The study by Järhult et al proposes surveillance in wild birds as a measure to understand the resistance situation in nature and to monitor it over time. Strategies to lower environmental levels of OC include improved sewage treatment and, more importantly, a prudent use of antivirals.

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    Mass culling of avian hosts has been the long-standing practice for influenza control within the avian reservoir. This study combines population genetics and epidemiological influenza dynamics in a mathematical model to explore the epidemiological and evolutionary repercussions of mass avian culling – concluding that mass culling achieves a short-term benefit at the expense of several long-term detriments.

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    Outbreaks of avian influenza caused by infection with low pathogenic H9N2 viruses have occurred in poultry, resulting in serious economic losses in Asia and the Middle East. It has been diffucult to eradicate the H9N2 virus because of its low pathogenicity, frequently causing inapparent infection. It is important for the control of AI to assess whether the H9N2 virus acquires pathogenicity as H5 and H7 viruses. In this study by Soda et al, they investigated whether a non-pathogenic H9N2 virus acquires pathogenicity in chickens when a pair of di-basic amino acid residues is introduced at the cleavage site of its HA molecule.

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    The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. This study examines the prospect of H5N1 jumping from poultry to donkeys, and highlights the need for surveillance of H5N1 in animals in the vicinity of backyard poultry units. The study was originally published in the Journal of Biomedical Science.

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    Increased transportation of live pigs appears to have driven an increase in the diversity of swine influenza viruses found in the animals in Hong Kong over the last three decades, according to a new study. In the longest study of its kind, Duke-NUS Graduate Medical School researchers found that swine viruses crossed geographic borders and mixed with local viruses, increasing their diversity.

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Documents

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